Dataset of mouse hippocampus profiled by LC MS MS for

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UPLCQTOFMSbased metabolomic analysis of the serum of

Sep 13,2017 Dataset of mouse hippocampus profiled by LC MS MS for#0183;In the present study,an acute repetitive HPC mouse model was established,and the serum metabolites were profiled using ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOFMS),in conjunctionUHPLC-QTOFMS-Based Metabolomic Analysis of the HippocampusJan 10,2019 Dataset of mouse hippocampus profiled by LC MS MS for#0183;Ultra-high liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-QTOFMS) in combination with univariate and multivariate statistical analyses was employed to deciphering metabolic changes associated with HPC in hippocampus tissue.MetaboAnalyst 3.0 was used to construct HPC related metabolic pathways.UHPLC-QTOFMS-Based Metabolomic Analysis of the HippocampusJan 10,2019 Dataset of mouse hippocampus profiled by LC MS MS for#0183;Ultra-high liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-QTOFMS) in combination with univariate and multivariate statistical analyses was employed to deciphering metabolic changes associated with HPC in hippocampus tissue.MetaboAnalyst 3.0 was used to construct HPC related metabolic pathways.

Tau and other proteins found in Alzheimers disease spinal

21 hours ago Dataset of mouse hippocampus profiled by LC MS MS for#0183;LC-MS/MS analysis The concentrated peptide mix was reconstituted in a solution of 2% acetonitrile (ACN) and 2% formic acid for MS analysis.Peptides were eluted from the ES802 column (75 m Dataset of mouse hippocampus profiled by LC MS MS for#215; 25 cm; Thermo Fisher) at 300 nl/min using a Dionex UltiMate 3000 nano LC system with a 90-min gradient from 2 to 35% buffer B (100% acetonitrile and 0.1 Stitching the synapse Cross-linking mass spectrometry Synaptic transmission is the predominant form of communication in the brain.It requires functionally specialized molecular machineries constituted by thousands of interacting synaptic proteins.Here,we made use of recent advances in cross-linking mass spectrometry (XL-MS) in combination with biochemical and computational approaches to reveal the architecture and assembly of synaptic protein Spatially-Resolved Exploration of the Mouse BrainFigure S-1 MALDI-MS profile of SPE-fractionated N-glycans from a single 100 Dataset of mouse hippocampus profiled by LC MS MS for#181;m section of the mouse brain.Figure S-2 LC/MS profile of 250+ N-glycan species identified from the mouse hippocampus.Figure S-3 Glycan relative abundances in the mouse hippocampus,broken down by a) biosynthetic class and b) degree of fucosylation.

Some results are removed in response to a notice of local law requirement.For more information,please see here.Previous123456NextQuantitative proteomic profiling reveals novel region

Quantitative proteomic profiling reveals novel region-specific markers in the adult mouse brain Proteomics .2014 Feb;14(2-3):241-61.doi 10.1002/pmic.201300196.Some results are removed in response to a notice of local law requirement.For more information,please see here.12345NextQuantitative proteomic analysis of mouse hippocampus in Quantitative proteomic analysis was used to identify the proteins associated with a formation of fear memory in mice.The proteins from the hippocampal region were isolated from three groups of trained aminals representing aquisition,consolidation and retrieval of a contextual fear conditioning.The samples were digested by trypsin,analyzed by LC-MSMS followed by protein identification and Slow Oscillations in the Mouse Hippocampus Entrained by Apr 23,2014 Dataset of mouse hippocampus profiled by LC MS MS for#0183;Different types of network oscillations occur in different behavioral,cognitive,or vigilance states.The rodent hippocampus expresses prominent oscillations at frequencies between 4 and 12 Hz,which are superimposed by phase-coupled oscillations (30100 Hz).These patterns entrain multineuronal activity over large distances and have been implicated in sensory information

Slow Oscillations in the Mouse Hippocampus Entrained by

Apr 23,2014 Dataset of mouse hippocampus profiled by LC MS MS for#0183;Different types of network oscillations occur in different behavioral,cognitive,or vigilance states.The rodent hippocampus expresses prominent oscillations at frequencies between 4 and 12 Hz,which are superimposed by phase-coupled oscillations (30100 Hz).These patterns entrain multineuronal activity over large distances and have been implicated in sensory informationSex differences in the molecular signature of the Mar 16,2017 Dataset of mouse hippocampus profiled by LC MS MS for#0183;The LC-MS/MS dataset supporting the conclusions of this article is available in the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD004496.Additionally,spectral count data and LC-MS/MS sample metadata are provided as Supporting Information (see Additional files 3 and 4).Quantitative proteomic profiling reveals novel region Quantitative proteomic profiling reveals novel region-specific markers in the adult mouse brain Proteomics .2014 Feb;14(2-3):241-61.doi 10.1002/pmic.201300196.

Quantitative proteomic analysis of mouse hippocampus in

Quantitative proteomic analysis was used to identify the proteins associated with a formation of fear memory in mice.The proteins from the hippocampal region were isolated from three groups of trained aminals representing aquisition,consolidation and retrieval of a contextual fear conditioning.The samples were digested by trypsin,analyzed by LC-MSMS followed by protein identification and Proteomic analysis of 14-3-3 zeta binding proteins in the The present studied used recombinant Histagged 14-3-3 to identify potentially novel 14-3-3 binding proteins in the mouse hippocampus.Using a proteomic LC-MS/MS approach we identified a number of previously known 14-3-3 binding proteins,including other 14-3-3 isoforms,and a series of potentially new 14-3-3 binding proteins.Proteomic analysis of 14-3-3 zeta binding proteins in the The present studied used recombinant Histagged 14-3-3 to identify potentially novel 14-3-3 binding proteins in the mouse hippocampus.Using a proteomic LC-MS/MS approach we identified a number of previously known 14-3-3 binding proteins,including other 14-3-3 isoforms,and a series of potentially new 14-3-3 binding proteins.

PXD002250 - proteome and pathway effects of chronic

Proteome and pathway effects of chronic haoperidol treatment in mouse hippocampus.Ontology highlight.ABSTRACT Seven male C57BL/6 mice,at 10 weeks of age,were injected daily intraperitoneally with 0.5 mg/kg of haloperidol,for 28 days.A control group of six animals was injected with vehicle only (saline). LC-MS/MS analysis of O Mass spectrometrical analysis of the mitochondrial carrier Spots from the third-dimensional gel electrophoresis were in-gel digested with trypsin,chymotrypsin and subtilisin.Subsequently,peptides were analyzed by nano-ESI-LC-MS/MS using collision-induced dissociation and electron transfer dissociation modes.Modiro{\texttrademark} v1.1 along with Mascot v2.2 software was used for data handling.Mass spectrometrical analysis of the mitochondrial carrier Spots from the third-dimensional gel electrophoresis were in-gel digested with trypsin,chymotrypsin and subtilisin.Subsequently,peptides were analyzed by nano-ESI-LC-MS/MS using collision-induced dissociation and electron transfer dissociation modes.Modiro{\texttrademark} v1.1 along with Mascot v2.2 software was used for data handling.

Invariable stoichiometry of ribosomal proteins in mouse

Nov 05,2019 Dataset of mouse hippocampus profiled by LC MS MS for#0183;LC-MS/MS in Data Analysis.LC-MS/MS experiments were performed on the same day for all age groups of both biological replicates; hence some samples (e.g.,from 4- and 7-mo-old mice) were stored longer than those from 3-wk- and 12-mo-old mice.Ingenuity Pathway Analysis of metabolomics dataFollow-up experiments consisted of 1D and 2D NMR and LC/MS structure elucidation and addition of standards to the samples.Once metabolites had been assigned,several were noted to have arisen from gut micro flora.These were excluded from cross platform pathway mapping because no transcriptomics data was gathered from the gutIngenuity Pathway Analysis of metabolomics dataFollow-up experiments consisted of 1D and 2D NMR and LC/MS structure elucidation and addition of standards to the samples.Once metabolites had been assigned,several were noted to have arisen from gut micro flora.These were excluded from cross platform pathway mapping because no transcriptomics data was gathered from the gut

Indepth protein profiling of the postsynaptic density

Sep 11,2014 Dataset of mouse hippocampus profiled by LC MS MS for#0183;Using ionmobility enhanced dataindependent labelfree LCMS/MS,we established a reference proteome of crude synaptosomes,synaptic junctions,and PSD derived from mouse hippocampus including TOP3based absolute quantification values for identified proteins.Indepth protein profiling of the postsynaptic density Nov 01,2014 Dataset of mouse hippocampus profiled by LC MS MS for#0183;Using ionmobility enhanced dataindependent labelfree LCMS/MS,we established a reference proteome of crude synaptosomes,synaptic junctions,and PSD derived from mouse hippocampus including TOP3based absolute quantification values for identified proteins.Indepth protein profiling of the postsynaptic density For LCMS analysis,the same subcellular fractionation protocol was used as described above to obtain synaptosomal membranes (P3).P3 was resuspended in 5 mM Tris/HCl,pH 8.1,and centrifuged in another sucrose density gradient (0.8/1.0/1.2 M) for 2 h at 85 000 Dataset of mouse hippocampus profiled by LC MS MS for#215; g for additional purification.Synaptic junctions (SJ) were collected from the 1.0/1.2 border,resuspended in Tris buffer

Indepth protein profiling of the postsynaptic density

For LCMS analysis,the same subcellular fractionation protocol was used as described above to obtain synaptosomal membranes (P3).P3 was resuspended in 5 mM Tris/HCl,pH 8.1,and centrifuged in another sucrose density gradient (0.8/1.0/1.2 M) for 2 h at 85 000 Dataset of mouse hippocampus profiled by LC MS MS for#215; g for additional purification.Synaptic junctions (SJ) were collected from the 1.0/1.2 border,resuspended in Tris buffer Hippocampal 7-Hydroxylated Neurosteroids Are Raised by Oct 23,2020 Dataset of mouse hippocampus profiled by LC MS MS for#0183;These observations in LC-MS/MS analysis,together with the impaired remote memory performance in Cyp7b1 KO mice (Figures 2E and 2F) and their decreased spine densities ,suggest that training-induced 7-OH-Preg and/or 7-OH-DHEA in the hippocampus could be responsible for the spine remodeling and the formation of remote memory.Frontiers Phosphoproteome Profiling Reveals Circadian Mar 22,2017 Dataset of mouse hippocampus profiled by LC MS MS for#0183;Phosphopeptides were eluted from the beads using 200 L of 10% (v/v) ammonia solution for 15 min and sonicated for another 15 min at 4 Dataset of mouse hippocampus profiled by LC MS MS for#176;C.After centrifugation,the retrieved supernatants were collected,acidified with 10% (v/v) TFA,desalted with in-house C18 desalting cartridges,and dried in a speed-vac prior to LC-MS analysis.LC-MS Analyses

Frontiers Phosphoproteome Profiling Reveals Circadian

Mar 22,2017 Dataset of mouse hippocampus profiled by LC MS MS for#0183;Phosphopeptides were eluted from the beads using 200 L of 10% (v/v) ammonia solution for 15 min and sonicated for another 15 min at 4 Dataset of mouse hippocampus profiled by LC MS MS for#176;C.After centrifugation,the retrieved supernatants were collected,acidified with 10% (v/v) TFA,desalted with in-house C18 desalting cartridges,and dried in a speed-vac prior to LC-MS analysis.LC-MS AnalysesDataset of mouse hippocampus profiled by LCMS/MS for This dataset reports on the analysis of mouse hippocampus by LCMS/MS,from mice fed a diet that was either deficient in n-3 FA (n-3 Def) or sufficient in n-3 FA (n-3 Adq).Label free quantitative (LFQ) analysis of the mass spectrometry data identified 1008 quantifiable proteins,115 of which were found to be differentially expressed between Dataset of mouse hippocampus profiled by LCMS/MS for Dataset of mouse hippocampus profiled by LCMS/MS for

Comparative Study of Human and Mouse Postsynaptic

Oct 05,2012 Dataset of mouse hippocampus profiled by LC MS MS for#0183;Direct comparison of protein components from human and mouse excitatory synapses is important for determining the suitability of mice as models of human brain disease and to understand the evolution of the mammalian brain.The postsynaptic density is a highly complex set of proteins organized into molecular networks that play a central role in behavior and disease.Combination of FASP and StageTip-Based Fractionation Dataset on the mass spectrometry-based proteomic profiling of the kidney from wild type and the dystrophic mdx-4cv mouse model of X-linked muscular dystrophy.Data in Brief 2020 ,28 ,105067.DOI 10.1016/j.dib.2019.105067.Cited by 1Publish Year 2016Author Jane A.English,Caitriona Scaife,Akiko Harauma,Melanie Focking,Kieran Wynne,Gerard Cagney,ToruDataset of mouse hippocampus profiled by LCMS/MSData Article Dataset of mouse hippocampus proled by LCMS/MS for label-free quantitation Jane A.Englisha,b,Caitriona Scaifeb,Akiko Haraumac,Melanie Fockinga,Kieran Wynneb,Gerard Cagneyb,Toru Moriguchic,David R.Cottera a Department of Psychiatry,Royal College of Surgeons in Ireland,ERC Beaumont Hospital,Dublin,Ireland b Proteome Research Centre,School of Medicine and

Cited by 1Publish Year 2016Author Jane A.English,Caitriona Scaife,Akiko Harauma,Melanie Focking,Kieran Wynne,Gerard Cagney,ToruDataset of mouse hippocampus profiled by LC-MS/MS for

This dataset reports on the analysis of mouse hippocampus by LC-MS/MS,from mice fed a diet that was either deficient in n-3 FA (n-3 Def) or sufficient in n-3 FA (n-3 Adq).Label free quantitative (LFQ) analysis of the mass spectrometry data identified 1008 quantifiable proteins,115 of which were found to be differentially expressed between BLOC-1 deficiency causes alterations in amino acid profile Jul 12,2017 Dataset of mouse hippocampus profiled by LC MS MS for#0183;van Liempd,S.M.,Cabrera,D.,Lee,F.Y.et al.BLOC-1 deficiency causes alterations in amino acid profile and in phospholipid and adenosine metabolism in the postnatal mouse hippocampus.(PDF) Dataset of mouse hippocampus profiled by LC-MS/MS This dataset reports on the analysis of mouse hippocampus by LCMS/MS,from mice fed a diet that was either deficient in n-3 FA (n-3 Def) or sufficient in n-3 FA (n-3 Adq).

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